Table 4. Selected genes whose expression profiles differ between polar and apolar growth in PglaA-racAG18V versus PglaA-racA in a time- and carbon source-independent manner. Genes are ordered into different processes and functions.
| Predicted protein function* | Open reading frame code | Up/Down | Closest S. cerevisiae ortholog |
| (Phospho)lipid metabolism and signaling | |||
| dehydrogenase involved in sphingosin 1-phosphate breakdown | An01g09260 | ↑ | Hfd1 |
| Lysophospholipase, synthesis of glycerophosphocholine | An16g01880 | ↑ | |
| plasma membrane flippase transporting sphingoid long chain bases | An02g06440 | ↑ | Rsb1 |
| glycerophosphocholine phosphodiesterase, synthesis of phosphocholine | An18g03170 | ↑ | Gde1 |
| lanosterol 14A-demethylase | An11g02230 | ↓ | Erg11 |
| C-14 sterol reductase, ergosterol synthesis | An01g07000 | ↓ | Erg24 |
| choline/ethanolamine permease | An01g13290 | ↓ | Hnm1 |
| Calcium homeostasis and signaling | |||
| Ca2+/calmodulin dependent protein kinase | An02g05490 | ↓ | Cmk2 |
| Cell wall remodeling and integrity | |||
| endo-glucanase EglA | An14g02760 | ↑ | |
| endo-glucanase EglB | An16g06800 | ↑ | |
| endo-glucanase similar to Trichoderma reesei egl4 | An14g02670 | ↑ | |
| alpha-glucanosyltransferase AgtA (GPI-anchored) | An09g03100 | ↑ | |
| chitin synthase ChsL | An02g02340 | ↑ | Chs3 |
| chitin transglycosidase | An16g02850 | ↑ | Crh1 |
| chitinase | An01g05360 | ↑ | Cts2 |
| cell wall protein similar to A. nidulans PhiA | An14g01820 | ↑ | |
| cell wall protein with internal repeats | An12g10200 | ↑ | |
| cell wall protein (flocculin) | An12g00140 | ↑ | Flo11 |
| protein involved in β-1,3 glucan synthesis | An05g00130 | ↓ | Knh1 |
| α-1,2-mannosyltransferase | An04g06730 | ↓ | Mnn2 |
| Primary metabolism | |||
| isocitrate lyase AcuD | An01g09270 | ↑ | Icl1 |
| citrate lyase | An11g00510 | ↑ | |
| citrate lyase | An11g00530 | ↑ | |
| succinate dehydrogenase | An16g07150 | ↑ | Osm1 |
| aspartate transaminase, synthesis of glutamate | An16g05570 | ↑ | |
| acetyl-CoA carboxylase, synthesis of fatty acids | An12g04020 | ↑ | |
| homo-isocitrate dehydrogenase, synthesis of lysine | An15g02490 | ↓ | Lys12 |
| arginosuccinate synthetase, synthesis of arginine | An15g02340 | ↓ | Arg1 |
| acetylornithine aminotransferase, synthesis of arginine | An15g02360 | ↓ | Arg8 |
| arginyl-tRNA synthetase | An02g04880 | ↓ | |
| aspartic beta semi-aldehyde dehydrogenase, synthesis of threonine and methionine | An11g09510 | ↓ | Hom2 |
| homoserine kinase, synthesis of threonine | An17g02090 | ↓ | Thr1 |
| threonine synthase | An16g02520 | ↓ | Thr4 |
| phosphoribosylglycinamide formyltransferase, synthesis of purines | An02g02700 | ↓ | Ade8 |
| Secondary metabolism | |||
| polyketide synthase | An11g07310 | ↑ | |
| similar to plant zeaxanthin epoxidase ABA2 | An03g06500 | ↑ | |
| similar to enniatin synthase esyn1 of Fusarium scirpi | An13g03040 | ↑ | |
| similar to enoyl reductase LovC of the lovastatin biosynthesis A. terreus | An13g02940 | ↑ | |
| similar to enoyl reductase LovC of the lovastatin biosynthesis A. terreus | An09g01880 | ↑ | |
| similar to HC-toxin peptide synthase HTS of Cochliobolus carbonum | An16g06720 | ↑ | |
| Transporter | |||
| polyamine transporter | An11g07300 | ↑ | Tpo3 |
| polyamine transporter | An12g07400 | ↑ | Tpo3 |
| polyamine transporter | An13g03220 | ↓ | Tpo1 |
| vacuolar basic amino acid transporter | An06g00770 | ↑ | Vba5 |
| oligopeptide transporter | An11g01040 | ↓ | Opt1 |
| hexose transporter | An02g07610 | ↑ | Hxt5 |
| galactose transporter | An01g10970 | ↓ | Gal2 |
| low-affinity Fe(II) transporter of the plasma membrane | An16g06300 | ↓ | Fet4 |
| siderophore transporter | An03g03560 | ↓ | Arn1 |
| plasma membrane multidrug transporter | An07g01250 | ↑ | Pdr5 |
| multidrug transporter | An13g03060 | ↑ | Snq2 |
| Protein trafficking | |||
| protein kinase involved in exocytosis | An08g03360 | ↑ | Kin1 |
| Other signaling processes | |||
| zinc finger transcriptional repressor | An04g08620 | ↑ | Oaf3 |
| protein recruiting the SAGA complex to promoters | An07g04540 | ↑ | Cti6 |
| histidine kinase osmosensor | An14g02970 | ↑ | Sln1 |
| transcriptional regulator involved in nitrogen repression | An02g11830 | ↑ | Ure2 |
| transcription factor similar to A. nidulans MedA | An02g02150 | ↑ | |
| transcription factor | An02g06180 | ↑ | |
| transcription factor important for salt stress resistance | An12g09020 | ↑ | Hal9 |
| DNA damage checkpoint protein during replication | An03g06930 | ↓ | Rad24 |
| SUN family protein involved in replication | An08g07090 | ↓ | Sim1 |
| transcription factor important for Zn2+ homeostasis | An08g01860 | ↓ | Zap1 |
| alpha subunit of the translation initiation factor | An18g04650 | ↓ | Gcn3 |
| Others | |||
| pathogenesis-related protein | An08g05010 | ↑ | Pry1 |
| hypothetical aspergillosis allergen rAsp | An03g00770 | ↓ |
Genes up-regulated are indicated with ↑, genes down-regulated with ↓. Differential gene expression was evaluated by moderated t-statistics using the Limma package [82] with a FDR threshold at 0.05 [83]. Identical ORFs which are differentially expressed in PglaA-racAG18V and ΔracA are indicated in bold. Fold changes and statistical significance is given in Additional file 1 and 2.
*
: Protein functions were predicted based on information inferred from the Saccharomyces genome data base SGD (http://www.yeastgenome.org/) and the Aspergillus genome database AspGD (http://www.aspergillusgenome.org/).