Table 3. Analyses of molecular variance (AMOVA) of three loci by morphotypes (PL, LB and SB collected in 2005) and either location or sex.
Gene | Terms | d.f. | Sum of squares | Variance | Variation (%) | Fixation index | p-value |
D-loop* | Among morphs | 1 | 0.3 | 0 | 0.83 | FSC : −0.01 | ns. |
Among locations within morphs | 7 | 0.39 | 0 | −1.44 | FST : −0.01 | ns. | |
Within locations | 389 | 58.58 | 0.15 | 100.62 | FCT : 0.01 | **** | |
Total | 397 | 59.27 | 0.15 | ||||
Among morphs | 1 | 0.17 | 0 | 0.4 | FSC : −0.01 | ns. | |
Among sexes within morphs | 2 | 0.11 | 0 | −0.66 | FST : 0 | ns. | |
Within sexes | 393 | 58.17 | 0.15 | 100.26 | FCT : 0 | **** | |
Total | 396 | 58.45 | 0.15 | ||||
Cath2 | Among morphs | 2 | 4.56 | 0.02 | 12.64 | FSC : 0.03 | **** |
Among locations within morphs | 7 | 2.2 | 0.01 | 3.02 | FST : 0.16 | ** | |
Within locations | 253 | 42.18 | 0.17 | 84.34 | FCT : 0.13 | * | |
Total | 262 | 48.94 | 0.2 | ||||
Among morphs | 2 | 4.56 | 0.03 | 13.48 | FSC : 0.01 | **** | |
Among sexes within morphs | 2 | 0.45 | 0 | 0.47 | FST : 0.14 | ns. | |
Within sexes | 258 | 43.94 | 0.17 | 86.05 | FCT : 0.13 | **** | |
Total | 262 | 48.94 | 0.2 | ||||
MHCIIα | Among morphs | 2 | 50.76 | 0.22 | 63.2 | FSC : 0.03 | **** |
Among locations within morphs | 8 | 0.88 | 0 | −0.13 | FST : 0.63 | ns. | |
Within locations | 402 | 50.93 | 0.13 | 36.92 | FCT : 0.63 | *** | |
Total | 417 | 102.57 | 0.34 | ||||
Among morphs | 2 | 51.44 | 0.22 | 64.06 | FSC : −0.01 | **** | |
Among sexes within morphs | 2 | 0.04 | 0 | −0.33 | FST : 0.64 | ns. | |
Within sexes | 408 | 50.91 | 0.12 | 36.28 | FCT : 0.64 | **** | |
Total | 412 | 102.39 | 0.34 |
Only PL and SB were sequenced for the D-loop. d.f.: Degrees of freedom. Significance: ns. p>0.05,
p<0.05,
p<0.01,
p<0.001,
p<0.001.