Figure 2.
(a) High-performance liquid chromatography (HPLC) chromatograms and (b) representative high-resolution mass spectrometry (HRMS) spectra of purified Tn-MUC1 (compound 2) as well as T-MUC1 (compound 7), STn-MUC1 (compounds 3–6 containing Neu5Ac, Neu5Ac9N3, Neu5Gc, and Neu5AcN3, respectively), and ST-MUC1 (compounds 8–11 containing Neu5Ac, Neu5Ac9N3, Neu5Gc, and Neu5AcN3, respectively) glycopeptides synthesized by one-pot multienzyme (OPME) systems.