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One-pot four-enzyme galactosylation system for the synthesis of T-MUC1 glycopeptide from Tn-MUC1. Enzymes used: EcGalK, Escherichia coli galactokinase; BLUSP, Bifidobacterium longum UDP-sugar pyrophosphorylase; PmPpA, Pasteurella multocida inorganic pyrophosphatase; CjCgtBΔ30-His6) mutant, Campylobacter jejuni β1–3-galactosyltransferase mutant.
