Figure 2.

IFN-γ producing T cells are required for IFN-γ in BAL fluid. (a) WT mice were NK depleted (αNK1.1) or not (control Ig) and 1 day later treated i.n. with SEA. At 40 h, lung (top) and spleen (bottom) cells were stained for DX5 and NK1.1 to confirm depletion. (b) BAL fluid was collected at 40 and 64 h after SEA from NK depleted and control Ig treated mice to determine IFN-γ levels. Bar graph of ELISA data show Mean +/− SEM of IFN-γ (pg/ml). Data were combined from 2 independent experiments with n = 4 for NK depletion and n = 4 for control Ig. (c) BAL fluid was collected at 40 h after SEA from WT and TCRβδ^−/− mice and bar graph show Mean +/− SEM of IFN-γ (pg/ml). Data were combined from 3 independent experiments with n = 3 for vehicle and n = 9 for SEA. (d – f) Nylon wool column enriched WT or IFN-γ^−/− deficient T cells (~10⁶) were separately transferred into TCRβδ^−/− mice, and 1 day after transfer mice were treated i.n. with SEA or vehicle alone. 72 h after SEA, BAL cells were analyzed by NK1.1 and DX5 for NK cell percentages (d) and total numbers (e), and IFN-γ levels in BAL fluid were determined by ELISA (f). Bar graph shows Mean +/− SEM from 2 independent experiments with n = 3 for vehicle and n = 5 for SEA.