Table 1. Data production and mapping results for the three samples used.
Samples | YH | NA18532 | NA18555 |
Target region ( bp) | 4970558 | 4970558 | 4970558 |
Raw reads | 7988210 | 5377408 | 5457998 |
Raw data (Mb) | 719 | 484 | 491 |
Mapped reads | 7766430 | 4875514 | 4991960 |
Uniquely mapped reads | 7317777 | 4559340 | 4672521 |
Reads uniquely mapped to target | 4794113 | 3023307 | 3100770 |
Capture specificity | 65.51% | 66.31% | 66.36% |
Mean fold coverage depth (x) | 87.32 | 55.09 | 56.50 |
Percent bases with coverage ≥1x | 97.29 | 96.95 | 97.20 |
Percent bases with coverage ≥4x | 96.52 | 95.66 | 95.95 |
Percent bases with coverage ≥10x | 95.50 | 93.84 | 94.13 |
Target regions here refer to contiguous MHC regions, rather than to the region actually covered by the designed probes. Capture specificity is defined as the percentage of uniquely mapped reads aligning to the target region.