Figure 6. Effect of rab7 deletion on surface markers. (A) Surface SLC3A2 levels were measured by flow cytometry in live, purified T cells of the indicated genotypes following 24 h of incubation in complete medium (unstimulated) or following activation with CD3/CD28 antibodies (stimulated). A representative histogram and a graph of the mean ± SEM at least 3 independent experiments is shown. (B) Mean FSC ± SEM from the experiments in (A) is graphed. (C) Surface and total SLC3A2 expression was measured in splenic T cells from wild-type or rab7 TKO mice. Analysis was restricted to live (surface SLC3A2) or nonapoptotic (total SLC3A2) cells. Means ± SEM from 3 independent experiments are shown. Differences between surface and total SLC3A2 levels within a genotype did not reach statistical significance at p < 0.05. (D) Purified T cells from WT or rab7 TKO mice were activated with CD3 or CD28 antibodies for 65 h before staining with the indicated surface proteins and CD4/CD8; the analysis was restricted to live cells. The mean ± SEM from four independent experiments is graphed, a representative histogram is shown. T-tests were performed to compare either of the knockout T cells to wild-type (denoted by *) or rab7 TKO and atg5 TKO (denoted by ^#). * or ^#p < 0.05; **p < 0.01; ***p < 0.001.