Figure 7. DFMO has less effect on T. cruzi infection in MEF atg5 knockout cells. MEF cells (MEF WT) and atg5^−/− cells (MEF atg5 KO) were grown in the presence (+DFMO) or absence (Ctr) of 1 mM DFMO during 48 h and then were infected with T. cruzi of the CL Brener strain (MOI = 10) for 24 h in the same conditions. After fixation, parasites were detected by indirect immunofluorescence using a specific antibody followed by incubation with FITC-phalloidin to label cell perimeters. (A) Confocal images depict intracellular parasites (arrows) in each type of cell and condition. (B) Quantification of the percentage of infected cells at the indicated conditions. Data represent the mean ± SEM of at least two independent experiments (number of counted cells ≈100). Significantly different from control: ***p < 0.001, **p < 0.01. Scale bars: 10 μm.