Figure 5. Mono-Pt induces autophagic cell death in human ovarian carcinoma Caov-3 cells. (A) Caov-3 cells were treated with various concentrations of Mono-Pt and/or 1 or 2 mM 3-MA for 24 h. Cell death was measured by trypan blue dye exclusion assay. *p < 0.05, **p < 0.01. (B) The knockdown of BECN1 or ATG7 was confirmed by western blot. (C) Caov-3 cells were transfected with control siRNA, siRNA targeting BECN1 or siRNA targeting ATG7. After 48 h, cells were treated with 0, 3, 10 or 30 μM Mono-Pt for 24 h, and cell death was measured by trypan blue dye exclusion assay. *p < 0.05, **p < 0.01. (D and E) Caov-3 cells were treated with various concentrations of Mono-Pt and/or chloroquine (D) or bafilomycin A1 (E) for 24 h. Cell death was measured by trypan blue dye exclusion assay. *p < 0.05, **p < 0.01.