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. 2013 May 10;89(5):165–182. doi: 10.2183/pjab.89.165

Table 1.

Substrate specificity of P. putida DDC

Substrate Km (mM) kcat (s−1) kcat/Km (mM−1 s−1)
DOPA 9.2 (±1.9) × 10−2 1.8 (±0.1) × 100 2.1 (±0.4) × 101
5-HTP 9.3 (±2.3) × 10−1 9.5 (±0.6) × 10−2 1.0 (±0.2) × 10−1
Phe 8.8 (±1.9) × 100 2.3 (±0.2) × 10−1 2.7 (±0.4) × 10−2
Trp 8.8 (±2.1) × 100 4.4 (±0.4) × 10−3 5.1 (±0.8) × 10−4
Tyr     1.1 (±0.1) × 10−3
3-Methoxy-Tyr     6.3 (±0.3) × 10−4

The kcat values were calculated based on the reported Vmax values. The estimated molecular mass was 54 kDa.