. Author manuscript; available in PMC: 2013 Jul 25.

Published in final edited form as: Virology. 2011 Jan 31;412(1):196–210. doi: 10.1016/j.virol.2011.01.007

Table 2.

Primers used for site-directed mutagenesis of HPV18 E4 sequence.

Primer^a	Sequence (5′ to 3′)
T23I	GCTACAGCATCCCACCTACAGG
RXL1	CCACCGCCAGGCATAGGCGGCTGCACGACCTG
RXL2	CCGCCAGGAGGCCGCTGCGGCACGACCTGGAC
RXLD	CCACCGCCAGGCATCCGCGGCGGCACGACCTGGAC
Δ21-23	CTCAGCCTGCTCAACAGCCCACCTCACAGGATCCCC
S22N	GCTTGTTAAACAGCTACAACACACCCCCTCACCGT
^∞ T23I	GTTAAACAGCTACAGCATACCCCCTCACCG
^∞ RXL1	CCTACGGCCAGACATCGGCGGCTACACGACCTG
^∞ RXL2	GCCAGACGTCCGCTGCGACACGACCTG
^∞ RXLD	GACCTACGGCCAGACATCCGCGGCGACACGACCTGGAC

Nucleotides underlined denotes altered nucleotide.

^∞

Primers used to construct mutations in GST-HPV18E1^E4 coding sequence, recombinant adenovirus-HPV18 E1^E4 sequence and in HPV18 genomic DNA.