Fig. 4.

Influence of cytoplasmic and vacuolar calcium on the SV type channel activity. The records were obtained in the cytoplasm-out configuration at 76 mV (a, c) and 36 mV (e). a Channel activity recorded at 10 μM CaCl₂, 100 mM NaCl, 15 mM HEPES/Tris, pH 7 in the medium and 2 mM CaCl₂, 100 mM KCl, 15 mM MES/Tris, pH 5.8 in the pipette. b Histogram based on four records as in a. c Channel activity recorded at 1 μM CaCl₂, 100 mM NaCl, 15 mM HEPES/Tris, pH 7 in the medium and 2 mM CaCl₂, 100 mM KCl, 15 mM MES/Tris, pH 5.8 in the pipette. d Histogram based on four records as in c. e Channel activity recorded at 1 μM CaCl₂, 100 mM NaCl, 15 mM HEPES/Tris, pH 7 in the medium and 2 mM EGTA, 100 mM KCl, 15 mM MES/Tris, pH 5.8 in the pipette. f Histogram based on four records as in e