Table 3.

EDB CMAP areas at baseline, and weeks 1, 12, and 16 post Botox injection (2.5 MU/0.1 mL) for paralysis rating of both the fresh toxin (control) and toxin refrigerated for 72 hours, or 1, 2, 3, or 4 weeks (test) (mean ms×mV±SD)

There was no difference in the individual paralysis rates of the CMAP area 1 week post injection between the fresh-toxin groups (control) and all of the refrigerated-toxin groups (test). Furthermore, there were no statistical differences between the control and all five test groups, which shows that meaningful EDB paralysis with both the fresh toxin and the variously refrigerated toxin at 1 week post Botox injection (p<0.0001). Similarly, testing of the paralysis rate the fresh toxin control group and all of the individual test groups (i.e., 72 hours, and 1, 2, 3, and 4 weeks of refrigeration) revealed no significant differences between group pairs (p>0.05), decrease equally over time, with recovery from muscle paralysis beginning after 16 weeks. % paralysis is average value of individual percent paralysis of EDB CMAP area in each subject.

^*p<0.0001.

EDB CMAP: extensor digitorum brevis compound muscle action potential, FT: fresh toxin, hrs: hours, RT: refrigerated toxin, wk: week.