Fig. 7.

Upregulation of Wnt/β-catenin signalling in the nasal ciliary margin (CM) of E12.5 BAT-gal⁺; Foxg1^−/− compound mutants. Horizontal E12.5 retinal sections were studied by in situ hybridisation for Wnt2b (A–B′′) and Lef1 (C–D′′) followed by immunohistochemistry for β-galactosidase (β-gal) in controls (A,A′,C,C′) and compound mutants (B–B′′and D–D′′). Immunohistochemistry for Lef1 in controls (E,E′) and Foxg1^−/− mutants (F–F′). Panels (A,B′,C,D′,E,F′) correspond to the nasal (N) and (A′,B′′,C′,D′′,E′) to the temporal (T) side of the same eye. The curly brackets in (B) and (D) indicate the nasal retinal folds shown in high power in panels (B′) and (D′) respectively, while the dotted squares indicated the temporal retina shown in high power in panels (B′′) and (D′′) respectively. The arrow in (D′′) indicates the start of the retinal pigment epithelium (RPE) in the temporal mutant retina. The dotted square in (F) delineates the high power image of the nasal folded retina in (F′). Immunofluorescence for Pax6 (green) (G), β-galactosidase (magenta) (H) and Pax6/β-galactosidase (I) on E12.5 BAT-gal⁺; Foxg1^−/− compound mutant retinas. Scale bar in (A) corresponds to 50 μm and applies for panels (A,A′,B′,B′′,C,C′,D′,D′′,E,E′,F′); (B,D,F), 100 μm; (G–I), 20 μm. Abbreviations: l, lens.