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. 2013 Jul 17;33(29):11899–11915. doi: 10.1523/JNEUROSCI.1131-13.2013

Figure 2.

Figure 2.

Transplanted stem cell fate favors oligodendrocytic lineage and cells associate with axons in the CC. A, E, I, YFP+ NPCs extend processes that associate with NF200+ axons in the CC. B, C, F, G, J, K, N, R, V, AA, Complete eYFP colocalization is present with OL markers MBP (B, F, J, late myelin marker), MAG (N, R, V; in extruded processes, mid-developmental marker), and Olig2 (AA, early lineage marker), while MBP immunoreactive projections only interact with the NF200+ axons (C, G, K). M, O, Q, S, U, W, Conversely, eYFP (M, Q, U) and MAG (O, S, W) expression does not overlap with astrocytic marker GFAP. D, H, L, P, T, X, AA, Merged images. Y, Z, Singly stained eYFP (Y) and Olig2 (Z) immunopositivity. AB, Quantification of neuronal marker NeuN+, GFAP+, and Olig2+ cells shows 96.5 ± 0.6% oligodendroglial differentiation, with 1.3 ± 0.6% and 2.0 ± 0.4% neuronal and astroglial differentiation respectively (n = 9). Data are presented as means ± SEM.