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. 2013 Mar 14;6(3):295–319. doi: 10.3390/ph6030295

Figure 3.

Figure 3

Plate-reader assay to assess aptamer binding and internalization. For the binding experiments, cells were fixed to inhibit active transport before incubation with the RNA aptamers. Live cells were used for the internalization experiments. (A) Binding (left) and internalization (right) of A9g into PSMA-expressing prostate cancer cells. (B) Binding (left) and internalization (right) of E1 aptamer into rat HER2-expressing mammary carcinoma cells. Cells only (no RNA) controls were carried out for each condition. Fluorescence was measured using an Analyst HT plate reader. (*, p < 0.001).