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. 2013 Mar 14;6(3):295–319. doi: 10.3390/ph6030295

Figure 5.

Figure 5

Quantitative and ultrasensitive internalization method (“QUSIM”). (A) 96-well microplate NIR Odyssey imager scan of serial dilutions for binding aptamer-NIR conjugate (A9g-NIR) and mutant, non-binding sequence conjugate (A9g.6-NIR) (upper panel) and standard curves with linear regression for RNA aptamer quantification (lower panel). (B) Quantification of the amount of aptamer-NIR internalized into PC3(PSMA+) cells vs. PC3(PSMA–) cells. (C) Time-dependent cell uptake of binding aptamer (A9g-NIR) vs. non-binding (mutant) aptamer (A9g.6-NIR). (D) Kinetics of specific A9g internalization using one-phase association curve fit (R2 = 0.9924, k = 0.542 min−1, half-time = 1.278 min).