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. Author manuscript; available in PMC: 2013 Jul 25.
Published in final edited form as: Methods Mol Biol. 2012;884:53–69. doi: 10.1007/978-1-61779-848-1_4

Figure 2.

Figure 2

Injection and microscope setup. A conventional dissecting microscope is used with an epifluorescence halogen light source. A video camera is mounted to the microscope. A nanoliter injection system from WPI is employed. An air filter to the right suppresses dust and air currents at the injection station. A computer for control of the video camera and for video editing is partially pictured to the left. A homemade aluminum stage warmer is shown on the stage. Temperature is controlled with a Lauda circulating water bath. During surgery, the aluminum block is covered with a small rectangle of fresh spill paper.