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. Author manuscript; available in PMC: 2013 Jul 25.
Published in final edited form as: Exp Neurol. 2009 Feb 20;217(1):165–170. doi: 10.1016/j.expneurol.2009.02.004

Figure 4.

Figure 4

Double immunostaining for CD31 (red) and claudin-5 (green) at 72 hrs after ischemia. Claudin-5 staining was colocalized with the endothelial maker CD31. Claudin-5 signals were decreased in ischemic (peri-infarct) cortex compared to matching areas in contralateral brain. Loss of claudin-5 signals appeared to be ameliorated in CD47 knockout mice compared to wildtype mice, in spite of the fact that cell densities (DAPI, blue) and microvessel numbers (CD31, red) appeared similar. Con: contralateral, ipsi: ipsilateral. Scale bar: 50 μm.