Table 1. Overall coverage results.
|
Experiment 1 (BRCA MASTR v1.2) |
Experiment 2 (BRCA MASTR v2.0) |
Experiment 3 (BRCA MASTR v2.1) |
|||||||
|---|---|---|---|---|---|---|---|---|---|
| Run | R1 | R2 | R3 | R4 | R5 | R6 | R7 | R8 | R9 |
| Samples | 7 | 7 | 7 | 7 | 7 | 7 | 5 | 5 | 5 |
| Passed reads | 106 699 | 71 391 | 77 696 | 98 227 | 76 860 | 91 653 | 89 102 | 111 668 | 83 076 |
| BRCA-mapped reads (% of passed) | 106 303 (99.6%) | 70 953 (99.4%) | 77 339 (99.54%) | 97 778 (99.54%) | 76 559 (99.6%) | 91 421 (99.75%) | 88 699 (99.5%) | 110 724 (99.15%) | 82 718 (99.5%) |
| Coverage, mean [min, max] | 81.8 [5,201] | 50.9 [0,133] | 62.7 [8,157] | 81.6 [0,200] | 115 [5,498] | 138 [6,494] | 216 [43,595] | 269 [51,807] | 202 [47,610] |
| Coverage SD | 31.3 | 21.6 | 23.77 | 31.7 | 49.5 | 55.8 | 91.36 | 107.8 | 85.26 |
| Coverage fold difference to mean ratio 90%/95% | 1.98/2.77 | 1.95/2.39 | 1.86/2.34 | 1.91/2.23 | 1.81/2.11 | 1.82/2.43 | 1.68/2.09 | 1.49/1.74 | 1.69/2.04 |
| No. of bases< 38 (% of mapped) | 9430 (8.2%) | 28 947 (25.2%) | 15 238 (13.3%) | 5680 (4.9%) | 2895 (1.78%) | 3696 (2.28%) | 0 | 0 | 0 |
| No. of fragments< 38 | 106 | 318 | 178 | 74 | 10 | 14 | 0 | 0 | 0 |