Table 1.

Determination of half-wave reduction potentials.^a

Agent	Concentration	Half-wave reduction potential mV ± SE
5d	10 μM	−92 ± 7 mV
5a	10 μM	−156 ±1 mV
2-NBP	50 μM	−213 ± 2 mV
5e	10 μM	−245 ± 1 mV
Nitrofurazone	50 μM	−279 ± 4 mV
5c	10 μM	−286 ± 2 mV
Mitomycin C	10 μM	−307 ± 1 mV
Misonidazole	50 μM	−453 ± 3 mV
BG-M2	50 μM	−475 ± 2 mV
Metronidazole	50 μM	−611 ± 7 mV

^aPolarographic half-wave reduction potentials of agents in relation to four well known reference compounds; nitrofurazone a topical bactericide; mitomycin C, a cancer chemotherapeutic agent preferentially targeting hypoxic regions; misonidazole a radiosensitizer, ¹⁸F derivatives of which are used for oxygen deficient region imaging; and metronidazole an antibiotic targeting anaerobic bacteria and protozoa. 2-NBP and BG-M2 are prototype O⁶-BG prodrugs designed to target hypoxic tumors. Half-wave reduction potentials were measured versus an Ag/AgCl reference electrode. All values are the result of at least 3 determinations ± SE