Table 1.
Agent | Concentration | Half-wave reduction potential mV ± SE |
---|---|---|
5d | 10 μM | −92 ± 7 mV |
5a | 10 μM | −156 ±1 mV |
2-NBP | 50 μM | −213 ± 2 mV |
5e | 10 μM | −245 ± 1 mV |
Nitrofurazone | 50 μM | −279 ± 4 mV |
5c | 10 μM | −286 ± 2 mV |
Mitomycin C | 10 μM | −307 ± 1 mV |
Misonidazole | 50 μM | −453 ± 3 mV |
BG-M2 | 50 μM | −475 ± 2 mV |
Metronidazole | 50 μM | −611 ± 7 mV |
Polarographic half-wave reduction potentials of agents in relation to four well known reference compounds; nitrofurazone a topical bactericide; mitomycin C, a cancer chemotherapeutic agent preferentially targeting hypoxic regions; misonidazole a radiosensitizer, 18F derivatives of which are used for oxygen deficient region imaging; and metronidazole an antibiotic targeting anaerobic bacteria and protozoa. 2-NBP and BG-M2 are prototype O6-BG prodrugs designed to target hypoxic tumors. Half-wave reduction potentials were measured versus an Ag/AgCl reference electrode. All values are the result of at least 3 determinations ± SE