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. Author manuscript; available in PMC: 2014 Jul 15.
Published in final edited form as: J Immunol. 2013 Jun 14;191(2):640–649. doi: 10.4049/jimmunol.1203315

Figure 7. HuR regulates IL-17-induced gene expression in vivo.

Figure 7

(A) Total BAL and differential cell counts in samples from HuRflox/wt (HuR WT) and HuRflox/flox (HuR KO) mice expressing SP-CrtTA/tetO-CRE (n = 6 per group) fed with doxycycline for one week and then left unchallenged (Control) or challenged for 24 h by intranasal injection of IL-17 (0.5 µg). *P < 0.05 (two-tailed t-test). (B) Lung sections of saline (NT) and IL-17-challenged mice (as described in Fig. 7a), stained with hematoxylin and eosin. Magnification ×100. (C) Enzyme-linked immunosorbent assay of CXCL1 in BAL fluid from control or IL-17-challenged mice (as described in Fig. 7a). (D) Real-time PCR analysis of Cxcl1 in lung tissue from control or IL-17-treated mice (as described in Fig. 7a). Results are presented in arbitrary units relative to the expression of β-actin mRNA. *P < 0.05 (two-tailed t-test). Data are representative of two independent experiments (mean and s.d. in a, c, d).