Fig. 3.

Characteristics of skin-infiltrating T cells following Mantoux test (MT) induction. (a) Cutaneous lymphocyte antigen (CLA) expression on peripheral blood and normal skin blister CD3⁺ T cells. Peripheral blood mononuclear cells (PBMC) and blister cells were isolated and stained with anti-CD3, anti-CD4- and anti-CLA antibodies and analysed by flow cytometry. Samples were analysed by gating on the CD3⁺CD4⁺ CD45RA⁻ subset of cells within the live lymphocyte/lymphoblast gate. Gating strategy was used to exclude cell debris, but include lymphocyte blasts. Dot-plots showing CLA expression on CD3⁺ T cells isolated from the peripheral blood (PB) and a suction blister raised on day 3 following MT induction (b) PBMC (◊) and suction blister (SB) (▪) cells isolated from skin following MT induction were analysed using flow cytometry for the percentage of CD4⁺ memory T cells positive for CLA expression. The mean ± standard error of the mean of three experiments per time-point is shown.