Fig. 5.

Detection of intracytoplasmic and secreted interleukin (IL)-32 protein in cultured biliary epithelial cells (BECs). (a) Western blotting revealed that the culture medium as well as cell lysate of poly(I:C)-treated cultured cells contained IL-32 protein, but the level was faint in untreated cells (Non). As a positive control, recombinant IL-32 (rIL-32, 0·1 μg) was used. Semiquantitative analysis using NIH image analysis confirmed that the density of bands was up-regulated in cell lysate and culture medium by stimulation with poly(I:C). (b) Immunocytochemistry also demonstrated that IL-32 was expressed strongly in the poly(I:C)-stimulated BECs compared with unstimulated BECs (Non). Original magnification ×400.