Fig. 6.

Effect of 2-chlorodeoxyadenosine (CdA) on lipopolysaccharide (LPS)-induced dendritic cell (DC) maturation and tumour necrosis factor (TNF)-α release. Immature (Im)DC were treated with 10 μM CdA in the absence or presence of LPS (100 ng/ml) and immunostaining for maturation markers [CD86 and human leucocyte antigen D-related (HLA-DR)] was performed and analysed by flow cytometry. (a) Histograms are representative for four independent experiments. Data were normalized to isotype controls and percentage of positive cells and mean fluorescence intensity (MFI) are presented. Isotype controls (shaded) were included for each staining. (b) Data are presented as the percentage change in the MFI. (c) The release of TNF-α (pg/ml) was measured in the culture medium. Data are represented as mean ± standard error of the mean (n = 4). *P < 0·05; **P < 0·01; ***P < 0·001 versus untreated ImDC control.