FIG. 1.

The sequence features of BW001 on adhesion-inhibiting ability. B16 cells (4×10⁴ per well) were co-cultured with ODN at 6μg/mL for 40 minutes at 37°C. Nonadherent cells were slightly washed away with PBS and the number of adherent cells in four replicate wells was counted. Results were expressed as adherence rate±SD by columns. The average number of four replicate wells was used to calculate the adhesion ratio of each ODN compared to PBS control. (A) The influence of CG motif and phosphorothioate (PS) modification. BW002 was GC reversed form of BW001 partPSBW001 and NStailBW001 were partially PS-modified versions of BW001. The sequences in box were phosphodiester linkage, and the sequences outside the box were phosphorothioate linkage. (B) The influence of polyG tail. (polyA) BW001, (polyC) BW001, and (polyT) BW001 were the forms that replaced the ployG tail, with polyA, polyC, and polyT, respectively, at 3′ end as indicated with underline. (C) The influence of the position of polyG motif. The position of polyG motif is underlined. (D) The influence of the number of guanine bases in polyG tail. BW001-1∼BW001-6 are, respectively, the forms owning a gradually decreased numbers of guanine base in polyG tail. (E) The sequence-effect relationship of BW001-like ODN. The ODNs used were indicated in the figure. Three independent experiments were performed, and data from one representative experiment of three are shown. Unpaired 2-tailed Student's t-test was applied to analyze the data. **p<0.001 versus PBS.