FIG. 3.

The influence of BW001 on B16 cell adhesion ability in a plate coated by fibronectin (FN) or type 1 collagen. In one procedure, the 96-well plate was coated with FN at 10 μg/mL (A) or type 1 collagen at 18.25 μg/mL (B). Suspensions of 4×10⁴ B16 cells containing BW001 at 6μg/mL were added in each well and co-cultured for another 40 minutes. In another procedure, the 96-well culture plate was coated by a mixture containing FN at 10 μg/mL (C) or type 1 collagen at 18.25 μg/mL (D) with BW001 at 6 μg/mL and incubated at 37°C for 30 minutes, following which suspensions of 4×10⁴ B16 cells were added in each well. Nonadherent cells were slightly washed away with PBS and the average number of adherent cells in four replicate wells was counted. Three independent experiments were performed, and data from one representative experiment of three are shown. Unpaired 2-tailed Student's t-test was applied to analyze the data. *p<0.05 versus PBS; **p<0.001 versus PBS.