FIG. 5.

The influence of BW001 on B16 cell adhesion to lung tissue. PKH26-labeled B16 cells suspensions containing BW001 at 6 μg/mL was incubated on fresh cryostat sections of lung tissue at 37°C for 40 minutes. Then, nonadherent cells were slightly washed away and adherent cells were fixed. (A) Representative lung tissue sections were photographed under fluorescence microscopy (magnification 200×, open bars=6 μm). Adherent cells are indicated with arrows. (B) Adherent B16 cell numbers on lung tissue sections. Three independent experiments were performed, and data from one representative experiment of three are shown. Unpaired 2-tailed Student's t-test was applied to analyze the data. **p<0.001 versus PBS.