FIG. 9.
Lung metastasis colony formation. (A, B) On day 0, suspensions of 3.5×105 B16 cells in a volume of 100 μL containing indicated ODN (25 μg per mouse) were injected into lateral tail veins of female C57BL/6 mice. On day 21, mice were sacrificed, lung tissues were separated (A) and the melanoma colony numbers on lung surface were counted (B). (C, D) Female C57BL/6 mice were injected with BW001 (25 μg per mouse) into lateral tail vein 1 hour earlier than tumor challenge. Co-administration of tumor cell and ODN was used as control group. Lung tissue of each mouse was seperated (C) and melanoma colony numbers on lung surface were counted (D). Colony numbers of each mouse are represented by open circles. The geometric mean of each group is indicated by a horizontal bar. Statistical significance was determined using the nonparametric test; p values are shown between ODN and PBS.