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Effect of chronic hypoxia on BLS. U87-Luc cells were grown in 96-well plates either in normoxia or hypoxia (95% N2/5% CO2) for 5 d (phase contrast microscopy, (a)). BLI was performed on triplicate wells at 1, 3, and 5 d and quantified at 1 min (b). The impact of 5 d of hypoxia on cell number and BLS was compared quantitatively (c). For cells treated with hypoxia for 5 d, BLI was repeated 5 min and 24 h after re-oxygenation and quantified at 1 min (d).
