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In vivo BLI after acute systemic hypoxia and cessation of blood flow. A nude mouse with a U87-Luc thigh xenograft tumor underwent BLI (a) before transfer to a hypoxic chamber (95% N2/5% CO2) for 10 min followed by BLI in a sealed bag (b). After euthanasia, BLI was performed (c). 1 mL of air was then injected into the tumor and BLI was repeated (d).
