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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: J Biomol Screen. 2012 Nov 7;18(4):388–399. doi: 10.1177/1087057112466430

Figure 3. Validation of the high throughput MEKK2 activity assay.

Figure 3

The intrinsic ATPase activity assay was performed using a low volume 384-well plate format with a total enzyme reaction volume of 5 μl. IC50 variability experiments were performed with the semi-automated high throughput screening activity assay using two control inhibitors. IC50 determinations for sunitinib (■) and dasatinib (●) resulted in average IC50 values (nM) ± SD of 963 ± 178 and 1,656 ± 319, respectively. Data are representative of three independent experiments.