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MS-MLPA probe mix with and without HhaI enzyme (DNA sequencer - ABI 3130). Results for Case 1 - biopsy 1 block 2 and biopsy 2 blocks 3 and 4. Note 15 peaks in the control DNA sample with HhaI. Presence of a peak in biopsies 1 and 2 (blocks 3 and 4) not present in the control DNA is that of aberrantly methylated CDKN2B gene. Presence of peak for aberrantly methylated DAPK1 in biopsy 1 block 2 and biopsy 2 block 3 not present in the control DNA.
