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. Author manuscript; available in PMC: 2013 Jul 25.
Published in final edited form as: Methods Mol Biol. 2012;863:187–206. doi: 10.1007/978-1-61779-612-8_11

Figure 11.

Figure 11

Methylation Specific PCR (MSP) confirmation of aberrant methylation detected by MS-MLPA for CDKN2B. Lane 1: universal methylated control; Lane 2: universal unmethylated control; Lanes 3 & 4: normal control, note presence of only unmethylated PCR product; Lanes 5–16 span Cases 1–6. Note presence of methylated and unmethylated product, the latter indicating admixture of normal and papilloma cells; Lanes 17 & 18: negative control.