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. Author manuscript; available in PMC: 2013 Jul 25.
Published in final edited form as: Methods Mol Biol. 2012;863:187–206. doi: 10.1007/978-1-61779-612-8_11

Figure 9.

Figure 9

Real-time polymerase chain reaction (PCR) with methylation-specific PCR (MSP) methylated (M) and unmethylated (U) primers for RARB for the control specimen (A), case 4 (B), and case 8 (C). Specific melting temperature peaks are seen for control U, M, and wild-type (W) DNA. The presence of the M melting temperature peak in case 4 indicates promoter hypermethylation of RARB, supported by MSP gel electrophoresis (Figure 4). The absence of an M melting temperature peak in case 8 is supported by MSP gel electrophoresis and MS multiplex ligation-dependent probe amplification.