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. Author manuscript; available in PMC: 2014 Jun 4.
Published in final edited form as: Sci Signal. 2013 Jun 4;6(278):ra43. doi: 10.1126/scisignal.2003389

Figure 5. TGF-β induced miR-192 expression is dependent on acetyltransferase activity and Akt activity.

Figure 5

(A to C) Effects of DNp300 on TGF-β induced miR-192 expression (A) and miR-192 promoter activity (Full-length and SacI constructs) (B and C) in MCs. MCs were transfected with pCMV or pCMV-p300DN (DNp300) and treated without (CTR) or with TGF-β for 24 hours. Data are means ± SEM from 3 experiments. (D to F) Effects of MK-2206 on TGF-β-induced miR-192 expression (D) and on miR-192 promoter activity (Full-length and SacI constructs) (E and F) in MCs. MCs were pre-treated with MK-2206 or vehicle DMSO and then treated with TGF-β. Data are means ± SEM from four experiments. (G) Effects of other histone acetyltransferases (CBP and PCAF) compared with p300 on miR-192 expression. MCs were transfected with DNp300, DNCBP, or DNPCAF, and treated with TGF-β (10 ng/ml) for 24 hours. Data are means ± SEM from three experiments. *P < 0.05 by one-way ANOVA followed by Tukey’s post-hoc test analysis.