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. 2013 Jul 19;6:33. doi: 10.1186/1756-6606-6-33

Table 1.

Primers for quantification of expression of GABAAR subunit genes in granule cells with real-time PCR

Gene NCBI mRNA reference sequence(s) Primers (5′-3′) Position Amplicon length (bp)
Gabra1
NM_010250.4
F: GAGCAGAAGTTGTCTATGAGTGG
1349, exon 7
164
 
 
R: GTGGAAGTGAGTCGTCATAACC
1512, exon 8
 
Gabra6
NM_001099641.1
F: CTACTCTGAAAATGTCAGTCGGATTC
364, exon 2
123
(variant 1)
R: CCAAAGCTGGTCACATAGATGTCT
486, exon 3
 
NM_008068.2
 
364, exon 2
123
 
(variant 2)
 
486, exon 3
 
Gabrb2
NM_008070.3
F: AGGGGCTACTTTGGGATTTGG
446, exon 2
108
 
 
R: TCTGTCCACCGTCTCTTTAACC
553, exon 3
 
Gabrb3
NM_008071.3
F: TTGCGGAGAAGACAGCCAAG
1128, exon 9
100
(variant 1)
R: TGAACATCCATCGGTGCTAATAGG
1227, exon 10
 
NM_001038701.1
 
1060, exon 9
100
 
(variant 2)
 
1159, exon 10
 
Gabrd
NM_008072.2
F: GCCAGCATTGACCATATCTCAG
324, exon 3
169
 
 
R: CATTCACGATGAAGGTGTCAGG
492, exon 4
 
Gabrg2
NM_008073.2
F: CTTACATTCCCTGCACACTCATC
1181, exon 7
140
(variant 1)
R: AGATTTTCTGGCTATGGTGCTT
1320, exon 8
 
NM_177408.5
 
822, exon 7
140
  (variant 2)   961, exon 8