Table 1.

Primers for quantification of expression of GABA_AR subunit genes in granule cells with real-time PCR

Gene	NCBI mRNA reference sequence(s)	Primers (5′-3′)	Position	Amplicon length (bp)
Gabra1	NM_010250.4	F: GAGCAGAAGTTGTCTATGAGTGG	1349, exon 7	164
		R: GTGGAAGTGAGTCGTCATAACC	1512, exon 8
Gabra6	NM_001099641.1	F: CTACTCTGAAAATGTCAGTCGGATTC	364, exon 2	123
	(variant 1)	R: CCAAAGCTGGTCACATAGATGTCT	486, exon 3
	NM_008068.2		364, exon 2	123
	(variant 2)		486, exon 3
Gabrb2	NM_008070.3	F: AGGGGCTACTTTGGGATTTGG	446, exon 2	108
		R: TCTGTCCACCGTCTCTTTAACC	553, exon 3
Gabrb3	NM_008071.3	F: TTGCGGAGAAGACAGCCAAG	1128, exon 9	100
	(variant 1)	R: TGAACATCCATCGGTGCTAATAGG	1227, exon 10
	NM_001038701.1		1060, exon 9	100
	(variant 2)		1159, exon 10
Gabrd	NM_008072.2	F: GCCAGCATTGACCATATCTCAG	324, exon 3	169
		R: CATTCACGATGAAGGTGTCAGG	492, exon 4
Gabrg2	NM_008073.2	F: CTTACATTCCCTGCACACTCATC	1181, exon 7	140
	(variant 1)	R: AGATTTTCTGGCTATGGTGCTT	1320, exon 8
	NM_177408.5		822, exon 7	140
	(variant 2)		961, exon 8