Table 2.
Properties of standard curves and lack of signal in no-template controls
| Gene | Slope | Y-intercept | Efficiency (%) | Regression | Cq of no-template controls |
|---|---|---|---|---|---|
| Gabra1 |
−3.21 |
41.22 |
105 |
0.995 |
48.9 (primer dimer) |
| (Cq for the lowest unknown concentration was 38.3) | |||||
| −3.42 |
41.72 |
96 |
0.993 |
No signal |
|
| −3.19 |
41.57 |
106 |
0.999 |
No signal |
|
| |
−3.30 |
40.65 |
101 |
0.998 |
No signal |
| Gabra6 |
−3.39 |
41.55 |
97 |
0.995 |
No signal |
| −3.48 |
41.59 |
94 |
0.987 |
No signal |
|
| −3.45 |
41.91 |
95 |
0.997 |
No signal |
|
| |
−3.39 |
41.52 |
97 |
0.999 |
No signal |
| Gabrb2 |
−3.22 |
39.62 |
104 |
0.998 |
No signal |
| |
−3.35 |
39.35 |
99 |
0.991 |
No signal |
| Gabrb3 |
−3.44 |
40.22 |
95 |
0.999 |
No signal |
| |
−3.37 |
40.99 |
98 |
0.999 |
No signal |
| Gabrd |
−3.11 |
40.68 |
110 |
0.998 |
No signal |
| −3.18 |
40.44 |
106 |
0.999 |
No signal |
|
| −3.33 |
39.49 |
100 |
0.996 |
No signal |
|
| |
−3.33 |
39.66 |
100 |
0.999 |
No signal |
| Gabrg2 |
−3.28 |
39.03 |
102 |
0.999 |
No signal |
| −3.34 | 39.70 | 99 | 0.996 | No signal |