Table 2.
Antibodies used for cell sorting, flow cytometric analysis and T cell activation assays.
| Target molecule | Clone number | Fluorophore/tag | Manufacturer | Application |
|---|---|---|---|---|
| IFN-γ | 1-D1-K | none | Mabtech | ELISPOT |
| IFN-γ | 7-B6-1 | biotin | Mabtech | ELISPOT |
| IL-2 | IL-2-I/249 | none | Mabtech | ELISPOT |
| IL-2 | IL-2-II | biotin | Mabtech | ELISPOT |
| TNF | TNF3/4 | none | Mabtech | ELISPOT |
| TNF | TNF5 | biotin | Mabtech | ELISPOT |
| CD3 | SK7 | FITC | BD Bioscience | Flow cytometry |
| CD3 | OKT3 | none | In house | T cell activation |
| CD1b | BCD1b3.1 | none | In house | Neutralization |
| Isotype IgG1 | P3 | none | In house | Neutralization |
| CD4 | RPA-T4 | APC | BD Bioscience | Flow cytometry |
| CD8α | OKT8 | none | In house | Flow cytometry |
| CD8β | 2ST8.5H7 | none | Beckman Coulter | Flow cytometry |
| TRAV1-2 | 3C10 | PE | Biolegend | Flow cytometry |