Figure 3. The R263 mutation results in the generation of a loss-of-function allele.
(A) Rbm5 mRNA expression in the Rbm5WT/WT, Rbm5sda/sda and Rbm5+/− (heterozygous knockout) postnatal day 28 testes, as determined by qRT-PCR analysis and normalised to Hprt expression. (B) RBM5 protein in postnatal day 28 testes of the Rbm5WT/WT and Rbm5sda/sda mice. HPRT was used as loading control. (C) Schematic of the Rbm5 gene-trap knockout allele. SA: splice acceptor site, Neo: neomycin resistant marker, Poly-A: polyadenylation signal (D) Testicular atrophy in the Rbm5sda/− (compound heterozygous) and Rbm5sda/sda males compared to an Rbm5WT/WT littermate. (E) Periodic acid-Schiff stained testis section of a 10-week-old adult Rbm5sda/− male. PS: pachytene spermatocyte, rST: round spermatid; FA: fragmented acrosomes.