Figure 3. Parameter estimation from experimental data including FRAP.

(A) Experimental FRAP recovery curves for Bni1 (Solid blue line: average of 5 experiments. Light blue area: 95% confidence interval around average. Dashed red: fit to exponential). The fit curve is used to determine the time to half-recovery, therefore the data is normalized by the maximum value of the fit curve. Notice that the WT curve has a much shorter time to half recovery than the LatA-treated curve (time to half recovery indicated by dashed black lines). Also see video S1. (B) FRAP simulation time to half recovery for varying diffusion rates and (left) and (right). There is no change with , while for as goes to zero the curves approach the theoretical no-diffusion limit (dashed black). Also see video S2. (C) Left: Cartoon illustrating the Bni1 temperature sensitive mutant experiment performed in [41] and simulated in this paper. Right: Phase plane of actin cable half-life (color-coded) as a function of and (simulation of the experiment in [41]), with the curve representing 45 s (dotted black) and our model fit (dashed black). This phase plane represents the average of those generated for initial conditions corresponding to 10 different observed cells.