Table 2.
Primer/probe sequences used in gene expression assays
| Gene | Primer/Probe | Sequence (5′-3′) |
|---|---|---|
| β-actin |
Probe |
/56-FAM/ATG GAG AAG /ZEN/ATCTGGCACCACACCTT/31ABkFQ/ |
| Forward |
ATCGAACACGGC ATT GTCACCAAC |
|
| Reverse |
AGT CAT CTT CTC ACGGTTGGCCTT |
|
| IL-1β |
Probe |
/56′FAM/AAA GAG CCT /ZEN/GGTGCTGTCTGG CTC AGA/31ABkFQ/ |
| Forward |
TGATGCAGC CAT ACAGTCACAGGA |
|
| Reverse |
CAAAGCTCATGCGGAACACCACTT |
|
| IL-2 |
Probe |
/56-FAM/ACT CGTCAC /ZEN/AAA CAG TGCACCTGCTT/31ABkFQ/ |
| Forward |
TAACCTCAA CTC CTGCCACCACAA |
|
| Reverse |
TTG CTC CAG CTGTTGCTGTGTTTC |
|
| IL-6 |
Probe |
/56-FAM/AACCGT AGA /ZEN/AGTTGGCCTGCAGCT AA/31ABkFQ/ |
| Forward |
TCC AGA TGCTGAAGCGTAAGGGAA |
|
| Reverse |
AAGGGT GAG GTG ATT GTTGTGTGC |
|
| IL-10 |
Probe |
/56-FAM/ACCAGGTCC /ZEN/TTGCTG GAG GACTTT AA/31ABkFQ/ |
| Forward |
TTTCAAACCAAGGAC GAG CTGCAC |
|
| Reverse |
AACTGGATCATCTCGGACAAGGCT |
|
| IL-17a |
Probe |
/56-FAM/GTGAGGACA /ZEN/GGA ATA GCATTCCCA CA/31ABkFQ/ |
| Forward |
TCC CAT TCAGTT CCC ATC ACT GCT |
|
| Reverse |
TCGGTAGTTGGG CAT CCTGGATTT |
|
| TGF-β |
Probe |
/56-FAM/AGCAATAAT /ZEN/TCCTGGCGCTAC CTC AGC A/31ABkFQ/ |
| Forward |
AGCACGTGGAGCTGTACC AGA AAT |
|
| Reverse |
TCCAGTGACATC AAA GGA CAG CCA |
|
| Gag |
Probe |
/56-FAM/AAGAACAAC /ZEN/AAG CAG AAGCAA GAT TTGCAC/31ABkFQ/ |
| Forward |
CAG GGTGCGCTG CAG ATA AAG AAA |
|
| Reverse |
ATG CTC TAC ACT GCATCC TAG CTG |
|
| FoxP3 |
Probe |
/56-FAM/AGC CTA CAC /ZEN/AAA TGCTTTGTGCGG GT/31ABkFQ/ |
| Forward |
GGTTCACACGCATGTTTGCCTTCT |
|
| Reverse |
ACT CGA ATT CAT CCACGGTCCACA |
|
| RORγT | Probe |
/56-FAM/AGA AATGCC /ZEN/TTGCCG TAG GGATGT CT/31ABkFQ/ |
| Forward |
AACCGTTGCCAACACTGTCGATTG |
|
| Reverse | ACGCCATGCCACTCTACA ACT CAA |
FAM = 6-carboxyfluorescein; fluorophore; ZEN = 5′ quencher; ABkFQ = Iowa Black FQ; 3′ quencher.