Table 1.
High enrichment for alternative splicing pre- and post-DBS on and off stimulation
| Type | Category | Term | Count | % | p-value |
|---|---|---|---|---|---|
|
PD |
SP_PIR_KEYWORDS |
cytoplasm |
72 |
2.875 |
0.000 |
| |
GOTERM_CC_FAT |
GO:0043232 ~ intracellular non-membrane-bounded organelle |
57 |
2.205 |
0.000 |
| |
SP_PIR_KEYWORDS |
phosphoprotein |
128 |
4.952 |
0.000 |
| |
GOTERM_CC_FAT |
GO:0005819 ~ spindle |
11 |
0.426 |
0.000 |
| |
SP_PIR_KEYWORDS |
alternative splicing |
129 |
4.990 |
0.000 |
| |
UP_SEQ_FEATURE |
splice variant |
129 |
4.990 |
0.000 |
| |
GOTERM_CC_FAT |
GO:0015630 ~ microtubule cytoskeleton |
20 |
0.774 |
0.000 |
| |
GOTERM_CC_FAT |
GO:0005856 ~ cytoskeleton |
35 |
1.354 |
0.000 |
| |
GOTERM_CC_FAT |
GO:0044430 ~ cytoskeletal part |
26 |
1.006 |
0.000 |
| |
GOTERM_CC_FAT |
GO:0031974 ~ membrane-enclosed lumen |
40 |
1.547 |
0.001 |
| |
GOTERM_BP_FAT |
GO:0033554 ~ cellular response to stress |
19 |
0.735 |
0.001 |
| |
SP_PIR_KEYWORDS |
cytoskeleton |
20 |
0.774 |
0.001 |
| |
SP_PIR_KEYWORDS |
atp-binding |
31 |
1.199 |
0.002 |
| |
GOTERM_MF_FAT |
GO:0004674 ~ protein serine/threonine kinase activity |
15 |
0.580 |
0.003 |
| |
GOTERM_BP_FAT |
GO:0006259 ~ DNA metabolic process |
16 |
0.619 |
0.003 |
| |
GOTERM_CC_FAT |
GO:0070013 ~ intracellular organelle lumen |
36 |
1.393 |
0.003 |
| |
GOTERM_BP_FAT |
GO:0032092 ~ positive regulation of protein binding |
3 |
0.116 |
0.004 |
| |
GOTERM_CC_FAT |
GO:0005815 ~ microtubule organizing center |
10 |
0.387 |
0.004 |
| |
GOTERM_MF_FAT |
GO:0000166 ~ nucleotide binding |
46 |
1.779 |
0.005 |
| |
GOTERM_CC_FAT |
GO: 0005694 ~ chromosome |
14 |
0.542 |
0.005 |
| |
GOTERM_CC_FAT |
GO:0043233 ~ organelle lumen |
36 |
1.393 |
0.005 |
| |
SP_PIR_KEYWORDS |
acetylation |
50 |
1.934 |
0.005 |
| |
GOTERM_CC_FAT |
GO:0070161 ~ anchoring junction |
8 |
0.309 |
0.006 |
| |
GOTERM_MF_FAT |
GO:0005524 ~ ATP binding |
33 |
1.277 |
0.006 |
| |
SP_PIR_KEYWORDS |
nucleotide-binding |
35 |
1.354 |
0.006 |
| |
GOTERM_CC_FAT |
GO:0005813 ~ centrosome |
9 |
0.348 |
0.007 |
| |
GOTERM_MF_FAT |
GO:0032559 ~ adenyl ribonucleotide binding |
33 |
1.277 |
0.008 |
| |
SP_PIR_KEYWORDS |
transferase |
30 |
1.161 |
0.008 |
| |
GOTERM_BP_FAT |
GO:0030099 ~ myeloid cell differentiation |
6 |
2.232 |
0.008 |
| |
GOTERM_BP_FAT |
GO:0009057 ~ macromolecule catabolic process |
20 |
0.774 |
0.008 |
| |
GOTERM_CC_FAT |
GO:0019898 ~ extrinsic to membrane |
14 |
0.542 |
0.009 |
| |
GOTERM_MF_FAT |
GO:0030554 ~ adenyl nucleotide binding |
34 |
1.315 |
0.009 |
|
DBS |
UP_SEQ_FEATURE |
FEATURE splice variant |
115 |
53.488 |
0.000 |
| |
SP_PIR_KEYWORDS |
phosphoprotein |
112 |
52.093 |
0.000 |
| |
SP_PIR_KEYWORDS |
alternative splicing |
114 |
53.023 |
0.000 |
| |
UP_SEQ_FEATURE |
domain:EH 1 |
3 |
1.395 |
0.002 |
| |
UP_SEQ_FEATURE |
domain:EH 2 |
3 |
1.395 |
0.002 |
| |
GOTERM_CC_FAT |
GO:0005829 ~ cytosol |
25 |
11.628 |
0.007 |
|
OFF |
SP_PIR_KEYWORDS |
alternative splicing |
63 |
55.263 |
0.000 |
| UP_SEQ_FEATURE | splice variant | 61 | 53.509 | 0.001 |
The functional terms as well as sequence feature and domains detected as the highest enriched in spliced transcripts of leukocyte RNA extracted from blood samples of advanced PD patients’ pre-DBS and post-DBS ON- and following one hour OFF- stimulus (p < 0.01) are shown. Red: the repeatedly appearing term alternative splicing. Given are: the compared state (PD: patients pre-DBS compared with control volunteers, DBS: PD patients post-DBS on electrical stimulation as compared with pre-DBS state, OFF: PD patients post-DBS following one hour of electrical stimulation cessation. Category: the type of term, term: the name of the term (and Gene Ontology – GO- ID for GO terms), count: the number of spliced transcripts that are associated with the corresponding term, %: the percent of the spliced transcripts in the term, p-value: statistical significance.