Figure 7. Overexpression of bHLH 13 or bHLH 17 attenuated JA responses.

(A) Quantitative real-time PCR analysis of expression levels of bHLH13 and bHLH17 in 11-day-old seedlings of WT and transgenic plants overexpressing bHLH13 or bHLH17. Two individual transgenic lines for bHLH13 (13OE-3 and 13OE-7) and bHLH17 (17OE-2 and 17OE-4) were representatively used in analysis. ACTIN8 was used as the internal control. (B) Anthocyanin contents of the indicated 11-day-old seedlings grown on MS medium supplied with indicated concentrations of MeJA. FW, fresh weight. Error bars represent SE (n = 3). Asterisks represent Student's t-test significance compared with WT plants: *, P<0.05; **, P<0.01. (C) Phenotypes of 7-day-old (upper panel) and 11-day-old (bottom panel) seedlings of WT and 17OE-4 grown on MS medium supplied without (CK) or with indicated concentrations (µM) of MeJA. (D) Quantitative real-time PCR analysis of expression levels of DFR, LDOX and UF3GT in 11-day-old seedlings of WT and 17OE-4 grown on MS medium supplied without (CK) or with 15 µM MeJA (JA). ACTIN8 was used as the internal control. (E) Quantitative real-time PCR analysis of expression levels of VSP1, PDF1.2 and LOX2 in 11-day-old seedlings of WT, 13OE-7 and 17OE-4 treated with mock (CK) or with 100 µM MeJA (JA) for 6 hours. ACTIN8 was used as the internal control. (F) Root phenotypes of 7-day-old WT and 17OE-4 seedlings grown on MS medium supplied without (CK) or with 20 µM MeJA. (G) Root length of 11-day-old WT, bHLH13 and bHLH17 overexpression transgenic seedlings grown on MS medium containing indicated concentrations of MeJA. Error bars represent SE (n = 15). Asterisks represent Student's t-test significance compared with WT plants: **, P<0.01. (H) Flowering time of WT, bHLH13 and bHLH17 overexpression transgenic lines. Data shown are the means from 24 plants. Error bars represent SE. Asterisks represent Student's t-test significance compared with WT plants: **, P<0.01.