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Protein level changes in SHP1, SHP2, p16, CDK4 and Cyclin D1 in radioresistant cells. A549S1 and A549S2 cells were cultured for three months. Cellular protein levels of SHP1, SHP2, p16, CDK4 and Cyclin D1 were examined by western blot. GAPDH was used as an internal control (*P < 0.05; **P < 0.01 vs. A549 cells).
