Table 1.

Oligonucleotides used as primers for multiplex PCR in the present study

Bacterium target gene (protein)	Primer	Sequence 5′ - 3′	Amplicon size (bp)
Streptococcus uberis	GSub-S	TGA TTC CGA CTA CTA CGC TAG AT	723
pauA (plasminogen activator A)	GSub-AS	ATA CTT TGA GTT TCA CCG AGT TC
Streptococcus dysgalactiae	GSdys-S	GTG CAA CTG CAT CAC TAT GAG	279
16S rRNA	GSdys-AS	CGT CAC ATG GTG GAT TTT C
Streptococcus agalactiae	GSag-S	ATT GAT AAC GAC GGT GTT ACT GT	487
sklA3 (fibrinogen binding protein)	GSag-AS	CAT AGT AGC GTT CTG TAA TGA TGT C