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. 2013 Jul 18;55(1):53. doi: 10.1186/1751-0147-55-53

Table 2.

Evaluation of single phenotypic identification tests for Gram-positive, catalase-negative cocci

Bacterium n αH1 CAMP1 ESC1 KM1 NACL1 INU1 HIP1 LAP1 PYR1
Streptococcus uberis
58
3%
9%
100%
8%a
0%a
100%a
92%a
100%a
68%a
Streptococcus dysgalactiae
20
100%
0%
0%
0%b
0%b
0%b
0%b
100%b
0%b
Streptococcus agalactiae
9
0%
100%
0%
0%
0%
0%
100%
100%
0%
Viridans streptococci
17
88%
0%
53%
47%
0%
24%
24%
88%
6%
Enterococcus spp
3
1/3
0/3
3/3
3/3
1/3
2/3
1/3
2/3
1/3
Aerococcus viridans
30
87%
0%
100%
3%
73%
13%
100%
3%
27%
Lactococcus garvieae
12
67%
0%
100%
0%
0%
17%
92%
100%
83%
Lactococcus lactis 2 0/2 0/2 2/2 1/2 0/2 2/2 2/2 2/2 1/2

All strains were isolated from milk of cows with intrammamary infection and previously identified by 16S rRNA sequencing. Data is expressed as percentage (%) of strains showing a positive reaction in the corresponding phenotypic tests.

1Alpha-hemolysis (αH); CAMP reaction (CAMP); esculin hydrolysis (ESC); growth on kanamycin esculin azide agar (KM); growth on sodium chloride agar (NACL); inulin fermentation (INU); hippurate hydrolysis (HIP); leucine aminopeptidase activity (LAP); pyrrolidonyl peptidase activity (PYR).

aFor these analyses, 25 strains of Streptococcus uberis were used.

bFor these analyses, 13 strains of Streptococcus dysgalactiae were used.