Table 2.
Evaluation of single phenotypic identification tests for Gram-positive, catalase-negative cocci
| Bacterium | n | αH1 | CAMP1 | ESC1 | KM1 | NACL1 | INU1 | HIP1 | LAP1 | PYR1 |
|---|---|---|---|---|---|---|---|---|---|---|
|
Streptococcus uberis |
58 |
3% |
9% |
100% |
8%a |
0%a |
100%a |
92%a |
100%a |
68%a |
|
Streptococcus dysgalactiae |
20 |
100% |
0% |
0% |
0%b |
0%b |
0%b |
0%b |
100%b |
0%b |
|
Streptococcus agalactiae |
9 |
0% |
100% |
0% |
0% |
0% |
0% |
100% |
100% |
0% |
| Viridans streptococci |
17 |
88% |
0% |
53% |
47% |
0% |
24% |
24% |
88% |
6% |
|
Enterococcus spp |
3 |
1/3 |
0/3 |
3/3 |
3/3 |
1/3 |
2/3 |
1/3 |
2/3 |
1/3 |
|
Aerococcus viridans |
30 |
87% |
0% |
100% |
3% |
73% |
13% |
100% |
3% |
27% |
|
Lactococcus garvieae |
12 |
67% |
0% |
100% |
0% |
0% |
17% |
92% |
100% |
83% |
| Lactococcus lactis | 2 | 0/2 | 0/2 | 2/2 | 1/2 | 0/2 | 2/2 | 2/2 | 2/2 | 1/2 |
All strains were isolated from milk of cows with intrammamary infection and previously identified by 16S rRNA sequencing. Data is expressed as percentage (%) of strains showing a positive reaction in the corresponding phenotypic tests.
1Alpha-hemolysis (αH); CAMP reaction (CAMP); esculin hydrolysis (ESC); growth on kanamycin esculin azide agar (KM); growth on sodium chloride agar (NACL); inulin fermentation (INU); hippurate hydrolysis (HIP); leucine aminopeptidase activity (LAP); pyrrolidonyl peptidase activity (PYR).
aFor these analyses, 25 strains of Streptococcus uberis were used.
bFor these analyses, 13 strains of Streptococcus dysgalactiae were used.