Figure 4.

Mapping Reads Resulting from ChIP with CENH3 Antibodies along Maize Chromosome 9.

The mapping results of chromosome 9 in 10-kb windows in three samples are shown in the top half of the figure. The x axis marks the position on the chromosome, and the centromere region is indicated by the green box. The y axis indicates the number of reads mapping uniquely to each position, normalized as per million mapped reads. There is a significant peak in the sDic-15 sample, which is 723-kb long, spanning from 54,447,000 to 55,170,000 bp, and is marked with a magenta box comparing with the two control lines [“control” and “T3-5(+)”], which do not contain sDic-15. The bottom half of the figure is the detailed layout of the 723-kb region using IGV, a visualization tool. Four tracks represent the ChIP-Seq mapping results of “control” and “sDic-15,” and the distribution of genes and transposable elements (TEs), which are represented using gray bars with white arrowheads that indicate the direction of each TE in this region.