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. 2013 Jun 21;25(6):2002–2021. doi: 10.1105/tpc.113.111757

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© 2013 American Society of Plant Biologists. All rights reserved.

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Figure 2. — Expression of MPF3 during Flower Development in Physalis.

(A) MPF3 mRNA in situ hybridization in early floral development. The floral meristem (FM), primordia of calyx (calP), corolla (corP), androecium (andP), and gynoecium (gynP) are indicated by arrows. From left to right, MPF3-antisense was probed in the first five images, and MPF3-sense was probed in the last image. Bars = 50 μm.

(B) RNA gel blot analysis of MPF3 after flower formation. Total RNAs were isolated from the leaf (L), calyx, corolla, androecium, gynoecium, and fruit. The developmental stages were roughly determined as small buds (sB), large buds (lB), young flowers (yF), mature flowers (mF), young fruits (yFr), large fruits (lFr), and old fruits (oFr). Actin was reprobed after stripping off MPF3 and was used as a loading control.